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Image Search Results
Journal: Journal of neurochemistry
Article Title: Caffeine stimulates amyloid beta-peptide release from beta-amyloid precursor protein-transfected HEK293 cells.
doi: 10.1046/j.1471-4159.1997.69041580.x
Figure Lengend Snippet: FIG. 4. Immunocytochemical dem- onstration of ßAPP and RYR5 in HEK293ßApp75l cells. A and B, D and E, and G and H are double-immu- nofluorescence photomicrographs. A: RYR localization, using anti- bodies XA7 (1:20) and GAM-Cy3 (1:250). B: The same field as in A, stained with antibodies aB5 (1:200) and GAR-Cy2 (1:250). ßAPP accumulations are similar, but not identical, to RYR localiza- tion (arrows). C: A magnified area of cytoplasmic processes stained with anti-XA7 showing a radial-lin- ear pattern (arrows). D: Eccentric accumulations of /3APP (anti-aB5) colocalize with a Golgi marker (arrows) (anti-58K, 1:50) in E. F: Cellular ßAPP labeled with anti- 5a3/1G7. G: /3APP immunostain- ing pattern (aB5) is compared with the ER marker anti-PDI (1:200) in H. I: The ßAPP (aB5) pattern is re- distributed in brefeldin-pretreated cells. J and K: Cell surface /3APP was labeled with aB5 at 4°Cand internalized at 25°Cfor 7.5 min in J and 20 min in K. Bars = 10 pm.
Article Snippet: Conjugated secondary antibodies used for immunocytochemistry were 1:250 dilutions of goat anti-mouse CY3, biotinylated goat anti-rabbit,
Techniques: Staining, Marker, Labeling